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human thymus marathon ready cdna library  (TaKaRa)
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TaKaRa human thymus marathon ready cdna library
Fig. 6. Real-time PCR analysis of pca-1mRNA expression in normal human tissues. The tissue-specific expression of pca-1mRNA was determined by an SYBR Green I ^ based quantitative PCR assay in normalized <t>cDNA</t> samples derived from15 different human tissues.The expression levels of PCA-1transcript in the human tissues were normalized for the expression levels of the b-actin transcripts in the same cDNAs.1, brain; 2, thymus; 3, heart; 4, lung; 5, liver; 6, spleen; 7, pancreas; 8, smallintestine; 9, colon;10, kidney;11, prostate;12, testis;13, ovary;14, peripheral blood leukocyte;15, placenta. Columns, averages of three independent measurements per tissue.
Human Thymus Marathon Ready Cdna Library, supplied by TaKaRa, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Fig. 6. Real-time PCR analysis of pca-1mRNA expression in normal human tissues. The tissue-specific expression of pca-1mRNA was determined by an SYBR Green I ^ based quantitative PCR assay in normalized cDNA samples derived from15 different human tissues.The expression levels of PCA-1transcript in the human tissues were normalized for the expression levels of the b-actin transcripts in the same cDNAs.1, brain; 2, thymus; 3, heart; 4, lung; 5, liver; 6, spleen; 7, pancreas; 8, smallintestine; 9, colon;10, kidney;11, prostate;12, testis;13, ovary;14, peripheral blood leukocyte;15, placenta. Columns, averages of three independent measurements per tissue.

Journal: Clinical Cancer Research

Article Title: High Expression of a New Marker PCA-1 in Human Prostate Carcinoma

doi: 10.1158/1078-0432.ccr-05-0195

Figure Lengend Snippet: Fig. 6. Real-time PCR analysis of pca-1mRNA expression in normal human tissues. The tissue-specific expression of pca-1mRNA was determined by an SYBR Green I ^ based quantitative PCR assay in normalized cDNA samples derived from15 different human tissues.The expression levels of PCA-1transcript in the human tissues were normalized for the expression levels of the b-actin transcripts in the same cDNAs.1, brain; 2, thymus; 3, heart; 4, lung; 5, liver; 6, spleen; 7, pancreas; 8, smallintestine; 9, colon;10, kidney;11, prostate;12, testis;13, ovary;14, peripheral blood leukocyte;15, placenta. Columns, averages of three independent measurements per tissue.

Article Snippet: The 5V-RACE (rapid amplification of cDNA ends) method was done to isolate the 5V-end portion of the newly identified cDNAs (activator protein, CCATCCTAATACGACTCACTATAGGGC; PCA-1-RACE, CTGTTCCCACAGTAGACC) using the human thymus Marathon-ready cDNA library (BD Clontech, Palo Alto, CA).

Techniques: Real-time Polymerase Chain Reaction, Expressing, SYBR Green Assay, Derivative Assay